Alternative polyadenylation controls a transcriptional state that determines the rate of Polycomb silencing

Quantitative regulation of Arabidopsis FLC involves a transcription-coupled chromatin mechanism whereby the decision to polyadenylate at proximal or distal sites is linked to histone demethylation of adjacent chromatin. To analyse the polyadenylation site choice in an unbiased and strand-specific manner, we carried out Quant-seq analysis on total RNA from 7DAG seedlings in Col-0, fca-9, and fld-4. To increase coverage fld4 libraries were enriched using an in solution bait target capture approach.

拟南芥FLC（Arabidopsis FLC）的定量调控涉及一种转录偶联染色质机制，即多聚腺苷酸化的近端或远端位点选择过程与相邻染色质的组蛋白去甲基化存在关联。为以无偏倚且链特异性的方式分析多聚腺苷酸化位点选择，我们对哥伦比亚生态型0号（Col-0）、fca-9突变体及fld-4突变体中发芽后7天（7DAG）幼苗的总RNA开展了Quant-seq分析。为提升测序覆盖度，我们通过溶液内诱饵靶向捕获方法对fld-4的测序文库进行了富集。