Lolium rigidum Gaud.Bromus tectorum L. Transcriptome

Lolium rigidum Gaud. (L. rigidum, Poaceae, Rigid Ryegrass) is a widespread monocotyledonous weed distributed in natural populations all around the Mediterranean, the Middle East and the Indian-subcontinent. It is an important component of pastures and is a major weed in the pasture-cereal crop rotation system in Australia. The cross and multiple resistance of L. rigidum to different herbicide groups is well documented. L. rigidum plants were identified with target site resistance against seven different herbicide groups. In order to explore the molecular background of herbicide resistance RNAseq analysis of L. rigidum plants was performed using a NextSeq 500 (Illumina, USA) sequencing platform.Bromus tectorum L. (B. tectorum, Poaceae, Downy Brome) is an invasive annual weed found in arable fields of North America, central Asia and in many European countries. As a consequence of the use of herbicides numerous Bromus populations have been confirmed to be herbicide resistant. B. tectorum has a great plasticity to the resistant genotype maintenance through occasional out-crossing and by inducing genotypic variability and adaptation. The aim of our work was to identify herbicide target genes in B. tectorum. To this end Illumina sequencing on a NextSeq 500 platform and comprehensive bioinformatics analysis of B. tectorum samples was performed.Sorghum halepense L. (S. halepense, Poaceae, Johnsongrass) is a C4 perennial monocotyledonous weed. It occurs in all major agricultural areas in the warm regions of the world. The extensively growing rhizomes and the high rate of seed production contribute to successful invasion and difficult eradication of this weed. S. halepense populations show resistance to several classes of herbicide groups, including multiple resistance as well. In order to explore the molecular background of resistance to AHAS-inhibitors and to identify herbicide target genes RNAseq analysis of resistant individuals was performed using the Illumina NextSeq500 technology.硬直黑麦草（Lolium rigidum Gaud.，下称L. rigidum，禾本科（Poaceae），又称刚性黑麦草（Rigid Ryegrass））是一种广布的单子叶恶性杂草，其自然种群遍布地中海地区、中东及印度次大陆。该物种既是牧场植被的重要组成部分，同时也是澳大利亚牧草-谷类轮作系统中的主要恶性杂草。硬直黑麦草对不同除草剂类别的交互抗性与多重抗性已有大量文献记载，且该物种已被证实对7类不同除草剂具备靶标位点抗性。为探究其除草剂抗性的分子背景，研究人员依托美国Illumina公司NextSeq 500测序平台，对硬直黑麦草植株开展了RNAseq分析。 旱雀麦（Bromus tectorum L.，下称B. tectorum，禾本科（Poaceae），又称软雀麦（Downy Brome））是一种入侵性一年生杂草，分布于北美、中亚及诸多欧洲国家的耕地中。由于除草剂的长期施用，多个旱雀麦种群已被证实具备除草剂抗性。该物种可通过偶然异交以及诱导基因型变异与适应性，对抗性基因型的维持具备极强的可塑性。本研究的目标为鉴定旱雀麦中的除草剂靶标基因，为此研究人员依托NextSeq 500平台开展Illumina测序，并对旱雀麦样本进行了全面的生物信息学分析。 假高梁（Sorghum halepense L.，下称S. halepense，禾本科（Poaceae），又称约翰逊草（Johnsongrass））是一种C4型多年生单子叶杂草，遍布全球温暖区域的所有主要农业产区。其发达的根茎系统与极高的种子产率，促成了该杂草的成功入侵与难以根除的特性。假高梁种群对多类除草剂均表现出抗性，同时具备多重抗性特征。为探究其对AHAS抑制剂（AHAS-inhibitors）类除草剂的抗性分子背景并鉴定除草剂靶标基因，研究人员采用Illumina NextSeq500技术，对抗性个体开展了RNAseq分析。