Chromatin accessibility profile at single nuclei level of cells from mouse hippocampus. Chromatin accessibility profile at single nuclei level of cells from mouse hippocampus

We performed single-nucleus RNA-seq and chromatin accessibility analyses (snRNA-seq and ATAC-seq) in the hippocampus of wild-type (WT) mice and mice carrying amyloid precursor protein knock-in mutations (APPNL-G-F) in response to chronic wheel-running exercise. Overall design: 8-week old female WT and APPNL-G-F mice were put into cages with running wheel for voluntary exercise for 10 weeks. Mice from 4 different conditions (WT_RT, WT_EX, APP_RT, APP_EX) were subjected to single nuclei RNA and ATAC sequencing.

本研究针对野生型（WT）小鼠及携带淀粉样前体蛋白敲入突变（APPNL-G-F）的小鼠，在慢性转轮运动干预下，对其海马体开展了单细胞核RNA测序（snRNA-seq）与染色质可及性分析（ATAC-seq）。
实验设计概述：选取8周龄雌性野生型与APPNL-G-F小鼠，置于配备自主跑轮的饲养笼中进行10周的自主运动干预。本次实验共设置4组实验对象（WT_RT、WT_EX、APP_RT、APP_EX），分别对各组小鼠开展单细胞核RNA测序与ATAC测序。