An Investigation of Circulating microRNA Profile in Clinical Cases of Subarachnoid Hemorrhages of Hong Kong. Homo sapiens

circulating microRNA analysis in Subarachnoid hemorrhages (SAH) patients and Heathy controls circulating microRNA profiling has been shown for biomarker discovery for early disease detection in serum, which is stable and accessible. we describe our progress and propose a workflow to investigate and directly compare the circulating microRNA profiles of SAH patients and healthy individuals, in order to characterize potential biomarkers for SAH. Then we verified the top microRNA by quantitative PCR. Informatics analysis of KEGG provided important mechanistic insight to their potential involved pathways. Finally we demonstrated a possible workflow and initial results which indicated that circulating miRNAs can be potential biomarkers of SAH. Overall design: We analyzed circulating miRNA from healthy controls (n=20) and SAH patients (n=20) using the Affymetrix miRNA 3.0 platform. Array data was processed by Affymetrix ConsoleTool. No techinical replicates were performed.

蛛网膜下腔出血（Subarachnoid Hemorrhage, SAH）患者与健康对照人群的循环微小RNA（microRNA）分析：循环微小RNA谱已被证实可用于血清样本中疾病早期检测的生物标志物发现，且血清样本具备稳定性与可及性。本研究阐述了相关研究进展，并提出一套研究流程，用于探究并直接对比SAH患者与健康个体的循环微小RNA谱，以筛选SAH潜在的生物标志物。随后通过定量聚合酶链反应（quantitative PCR, qPCR）对筛选得到的差异表达最显著的微小RNA进行了验证。通过京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）开展的生物信息学分析，为这些微小RNA潜在参与的信号通路提供了重要的机制层面见解。最终本研究展示了一套可行的研究流程与初步结果，证实循环微小RNA可作为SAH的潜在生物标志物。
实验设计：本研究采用Affymetrix miRNA 3.0芯片平台，对20例健康对照个体（n=20）与20例SAH患者（n=20）的循环微小RNA进行了检测。芯片数据通过Affymetrix ConsoleTool进行处理。本研究未开展技术重复实验。