A Physiological Biomimetic Culture System for Pig and Human Heart Slices

We have developed and optimized a reliable medium-throughput culture system for pig and human heart slices as a platform for testing the efficacy of novel heart failure therapeutics and reliable testing of cardiotoxicity in a 3D heart model. Overall design: Temporal transcriptome analysis using RNAseq at day 2, 6, and 10 in culture confirmed overall maintenance of normal gene expression for up to 6 days, while over 500 transcripts were differentially regulated after 10 days.

本研究开发并优化了一套可靠的中通量培养体系（medium-throughput culture system），用于培养猪源与人类心脏薄片（heart slices），可作为新型心力衰竭治疗药物（heart failure therapeutics）的疗效测试平台，同时可在三维心脏模型（3D heart model）中可靠开展心脏毒性（cardiotoxicity）检测。
实验整体设计：在培养第2、6、10天通过RNA测序（RNAseq）开展时间维度转录组分析，结果证实该体系可维持猪源、人源心脏薄片的正常基因表达长达6天；培养至第10天时，已有超过500个转录本（transcripts）出现差异表达调控。