Additional file 1: of Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines

Figure S1. Expression of CD19/CD20, CD79A/B and RAG1/RAG2 in blood cancer cell lines. X axis: 164 blood cancer cell lines grouped base on the disease types (rectangle color bar below the X axis), each dot in X axis represent one cell line, and the Y axis, expression level (log2 value) of indicated genes. Figure S2. Clonal fraction (filtered by >30 reads) of B-ALL, multiple myeloma, diffuse large B cell lymphoma, Burkitt lymphoma, B cell lymphoma (unspecified) and mantle cell lymphoma base on IGH or IGHK/L. Blue color indicates the clonotype fraction of the most dominant clone, Red color indicate the clonotype fraction of second dominant clone, Yellow color indicate the third dominant clone, while any smaller subclones were aggregated and labelled in grey. Figure S3. Clonal fraction (filtered by >30 reads) of T-ALL and anaplastic large cell lymphoma base on TRCA or TRCB. Blue color indicates the clonotype fraction of dominant clone, red color inidicate the clonotype fraction of second dominant clone, while gray color indicate the third dominant clone. Figure S4. Heatmap showing the usage of IGK/L V genes (A), IGK/L J genes (B), and constant region (C) in 462 samples of EBV transformed normal B lymphocytes. Figure S5. The phylogenetic tree inferred based on the rearrangement of the CDR3 region of IGH, IGK and IGL of EBV transformed B lymphocyte samples ERR188025, ERR188358 and ERR188212. These three cell lines have much higher number of rearrangement types than the other B lymphocyte lines. Clonal Fraction (upper panel) and the read counts (lower panel) of the dominant clone of 462 samples of EBV transformed normal B lymphocytes. (ZIP 1290 kb)

补充图S1：血液癌细胞系中CD19/CD20、CD79A/B与RAG1/RAG2的表达水平。横轴：164株血液癌细胞系按疾病类型分组（横轴下方配有矩形色标标注分组信息），横轴上每个圆点代表一个细胞系；纵轴：目标基因的表达水平（以log2值计）。补充图S2：基于IGH或IGHK/L的B细胞急性淋巴细胞白血病（B-ALL）、多发性骨髓瘤、弥漫大B细胞淋巴瘤、伯基特淋巴瘤、未明确分型的B细胞淋巴瘤以及套细胞淋巴瘤的克隆分数（过滤条件：测序reads数>30）。其中蓝色代表优势克隆的克隆型占比，红色代表次优势克隆的克隆型占比，黄色代表第三优势克隆的克隆型占比，其余较小的亚克隆合并后以灰色标注。补充图S3：基于TRCA或TRCB的T细胞急性淋巴细胞白血病（T-ALL）与间变性大细胞淋巴瘤的克隆分数（过滤条件：测序reads数>30）。其中蓝色代表优势克隆的克隆型占比，红色代表次优势克隆的克隆型占比，灰色代表第三优势克隆的克隆型占比。补充图S4：热图展示了462例EB病毒（EBV）转化的正常B淋巴细胞样本中IGK/L V基因（A部分）、IGK/L J基因（B部分）以及恒定区（C部分）的使用情况。补充图S5：基于ERR188025、ERR188358与ERR188212三株EBV转化的B淋巴细胞样本的IGH、IGK及IGL的CDR3区域重排结果构建的系统发育树。该三株细胞系的重排类型数量远多于其余B淋巴细胞系。同时展示了462例EB病毒转化的正常B淋巴细胞样本的优势克隆的克隆分数（上图）与测序reads计数（下图）。（ZIP格式，1290 KB）