Gene-expression data of female wild-type (WT) and knock-out (KO) bone marrow derived macrophages (BMDMs) from C57BL/7 mice. Gene-expression data of female wild-type (WT) and knock-out (KO) bone marrow derived macrophages (BMDMs) from C57BL/7 mice

Leishmania are medically relevant protozoan parasites that cause leishmaniasis, a neglected tropical disease that can differently manifest depending on the species and the immune status of the host. Previously, it has been suggested that the presence of an endosymbiont double stranded RNA virus, Leishmania RNA Virus 1 (LRV1), within the parasite may leed to an exarcerbation of the disease outcome and represents an important factor for treatment failure and relapse. Here, we provide gene expression data of wild-type (WT) and various knock-out (KO) bone marrow derived macrophages (BMDMs) from mice (C57BL/6) in different conditions of infections and treatments. We included BMDMs from WT, Ifnar-/-, IFNg-/-, iNOs-/-, Nlrx1-/-, Nox2-/- and Prx5-/- mice. BMDMs were either infected with a human protozoan parasite Leishmania guyanensis with or without its endosymbiant dsRNA virus, LRV1 (LgyLRV1+ and LgyLRV1-, respectively). Alternatively BMDMs were treated with poly I:C, a synthetic dsRNA or a TLR2 agonist FSL1 to identify virus dependetn pathways or to explore the impact of co-exposure to additional agents, respectively. Overall design: Transcriptomic analysis of female WT and 6 KO BMDMs. A total of 62 samples were included in triplicates (total 186).

利什曼原虫（Leishmania）是具有医学重要性的原生动物寄生虫，可引发利什曼病——一种被忽视的热带病，其临床表现因寄生虫虫种与宿主免疫状态而异。既往研究提示，寄生虫体内的内共生双链RNA病毒——利什曼RNA病毒1（Leishmania RNA Virus 1, LRV1）的存在，可能加剧疾病进程，是治疗失败与复发的重要影响因素。本研究提供了C57BL/6小鼠来源的野生型（WT）及Ifnar-/-、IFNg-/-、iNOs-/-、Nlrx1-/-、Nox2-/-、Prx5-/-共6种基因敲除型骨髓来源巨噬细胞（bone marrow derived macrophages, BMDMs）在不同感染与处理条件下的基因表达数据。骨髓来源巨噬细胞分别感染带有或不带内共生dsRNA病毒LRV1的人类原生动物寄生虫圭亚那利什曼原虫（Leishmania guyanensis，分别记为LgyLRV1+与LgyLRV1-）；或是采用合成双链RNA聚肌胞苷酸（poly I:C）或TLR2激动剂FSL1处理巨噬细胞，以分别鉴定病毒依赖通路，或探究联合暴露于其他试剂的影响。整体实验设计：本研究对野生型及6种基因敲除型雌性小鼠骨髓来源巨噬细胞开展转录组分析，共纳入62组样本，每组设置3次生物学重复，总样本量为186。