Direct and indirect consequences of PAB1 deletion in the regulation of translation initiation, translation termination, and mRNA decay

Cytoplasmic poly(A)-binding protein (PABPC, yeast Pab1) is involved in various stages of mRNA post-transcriptional control, including translation initiation, translation termination, and mRNA decay regulation. Due to the multi-functional properties of PABPC, attempts to define a specific role for PABPC in vivo by deleting, depleting, overexpressing, or mutating the protein has led to conflicting models of PABPC's function. To specifically assess direct and indirect consequences of deleting PABPC, we generated and analyzed RNA-Seq, ribosome profiling, and mass spectrometry data from yeast cells lacking Pab1.

细胞质多聚腺苷酸结合蛋白（Cytoplasmic poly(A)-binding protein, PABPC，酵母中的同源蛋白为Pab1）参与mRNA转录后调控的多个阶段，涵盖翻译起始、翻译终止以及mRNA降解调控等过程。鉴于PABPC具备多重功能属性，既往通过敲除、耗竭、过表达或突变该蛋白以明确其在体内特定功能的研究，均得到了关于PABPC功能的相互矛盾的模型。为特异性评估敲除PABPC所引发的直接与间接效应，我们对缺失Pab1的酵母细胞的RNA测序（RNA-Seq）、核糖体谱分析（ribosome profiling）及质谱（mass spectrometry）数据进行了生成与分析。