Transcriptome analysis reveals the potential mechanism of MAP34-B target to Pasteurella multocida Original data

Pasteurella multocida is a prevalent zoonotic pathogen that causes significant damage to the poultry industry. It has been found that antimicrobial peptide MAP34-B exhibits strong antibacterial activity against Pasteurella multocida, although the specific mechanism of its antibacterial action remains unclear. To investigate the precise antibacterial mechanism of MAP34-B against Pasteurella multocida, Clinical strain HB03 of Pasteurella multocida, was conducted transcriptomic analysis on bacterial samples of strain HB03 treated with or without MAP34-B (47.4 µM) for 1 hour using RNA sequencing (RNA-seq).The results showed that, after treatment with MAP34-B, 281 differentially expressed genes were identified, including 161 upregulated genes and 120 downregulated genes. KEGG pathway enrichment analysis revealed that the Ribosome pathway had the highest proportion of affected genes. After treatment with MAP34-B, the expression levels of genes rps2, rps3, rps9, rps16, rpl3, rpl9, rpl22, and rpl23, were upregulated, which may affect bacterial protein synthesis. Additionally, the expression levels of membrane-associated genes, such as SecE, SecG, lolB, and ompR, were also altered, disrupting the integrity of the bacterial cell membrane. Therefore, the antibacterial effect of MAP34-B primarily involves its impact on bacterial cell membrane integrity and protein synthesis, providing a theoretical basis for the use of MAP34-B in the treatment of bacterial infections.

多杀性巴氏杆菌（Pasteurella multocida）是一种流行的人畜共患病原菌，对家禽养殖业造成严重危害。已有研究表明，抗菌肽（antimicrobial peptide）MAP34-B对多杀性巴氏杆菌具有较强的抗菌活性，但其具体抗菌作用机制尚不清楚。为探究MAP34-B对抗多杀性巴氏杆菌的精准抗菌机制，本研究以多杀性巴氏杆菌临床菌株HB03为研究对象，采用RNA测序（RNA-seq）技术，对经47.4 μM MAP34-B处理与未处理的HB03细菌样本进行了1小时的转录组分析。结果显示，经MAP34-B处理后，共鉴定出281个差异表达基因，其中上调基因161个，下调基因120个。KEGG通路富集分析表明，核糖体通路受影响的基因占比最高。经MAP34-B处理后，rps2、rps3、rps9、rps16、rpl3、rpl9、rpl22及rpl23等基因的表达水平上调，这可能会影响细菌的蛋白质合成过程。此外，SecE、SecG、lolB、ompR等膜相关基因的表达水平也发生了改变，破坏了细菌细胞膜的完整性。综上，MAP34-B的抗菌作用主要通过影响细菌细胞膜完整性与蛋白质合成途径实现，该研究为MAP34-B用于细菌性感染治疗提供了理论依据。