DataSheet2_Dynamics of the Zebrafish Skeleton in Three Dimensions During Juvenile and Adult Development.PDF

Zebrafish are a valuable model for normal vertebrate skeletogenesis and the study of myriad bone disorders. Bones grow, ossify and change shape throughout the zebrafish lifetime, and 3D technologies allow us to examine skeletogenic processes in detail through late developmental stages. To facilitate analysis of shape, orientation and tissue density of skeletal elements throughout ontogeny and adulthood, we generated a high-resolution skeletal reference dataset of wild-type zebrafish development. Using microCT technology, we produced 3D models of the skeletons of individuals ranging from 12 to 25 mm standard length (SL). We analyzed the dynamics of skeletal density and volume as they increase during juvenile and adult growth. Our resource allows anatomical comparisons between meristic units within an individual—e.g., we show that the vertebral canal width increases posteriorly along the spine. Further, structures may be compared between individuals at different body sizes: we highlight the shape changes that the lower jaw undergoes as fish mature from juvenile to adult. We show that even reproductively mature adult zebrafish (17–25 mm SL) continue to undergo substantial changes in skeletal morphology and composition with continued adult growth. We provide a segmented model of the adult skull and a series of interactive 3D PDFs at a range of key stages. These resources allow changes in the skeleton to be assessed quantitatively and qualitatively through late stages of development, and can serve as anatomical references for both research and education.

斑马鱼是研究正常脊椎动物骨发生以及多种骨骼疾病的珍贵模式生物。斑马鱼的骨骼会在其整个生命周期中生长、骨化并改变形态，而三维（3D）技术使我们能够细致解析发育晚期的骨发生过程。为便于分析骨骼结构在个体发育与成年阶段的形态、方位与组织密度，我们构建了野生型斑马鱼发育过程的高分辨率骨骼参考数据集。本研究利用显微计算机断层扫描（microCT）技术，构建了标准体长（SL）为12至25 mm的个体的骨骼三维模型。我们分析了幼体与成年生长阶段中骨骼密度与体积随生长增加的动态变化规律。本数据集支持个体内分节结构单元的解剖学比较——例如我们发现，椎管宽度沿脊柱向后方逐渐增加。此外，还可对不同体长个体间的骨骼结构进行比较：本研究揭示了斑马鱼从幼体成熟至成体过程中下颌所经历的形态变化。我们证实，即使是性成熟的成年斑马鱼（标准体长17–25 mm SL），在成年生长阶段仍会发生骨骼形态与组成的显著变化。本研究提供了成体颅骨的三维分割模型，以及一系列覆盖关键发育阶段的交互式三维（3D）PDF文件。这些资源支持在发育晚期阶段对骨骼变化进行定量与定性评估，可同时作为科研与教学的解剖学参考资料。