Single cell RNA sequencing of lung myeloid cells from aged (20-22 months old) mice treated with Isotype or anti-IFNAR1 antibody for 1 day or 1 week

Monocytes and neutrophils are an important subset of myeloid cells in the lung. However, little is known about how aging affects these lung cells, and the role of Type I interferon signaling. Here, we treat aged (20-22 months old) mice with 500ug anti-IFNAR1 antibody (Clone MAR1-5A3 from BioXcell) intraperitoneally (i.p.) once or for 1 week (total 4 i.p. injections, one every other day) and sorted CD45+ CD11b+ Gr1+ cells from the lung the day after the last injection. We performed single cell RNA sequencing to identify the role of Type I Interferon in these cells from the aging mouse lung. We use 10x Genomics platform to prepare scRNA-seq libraries to detail the different gene expression between CD45+ CD11b+ Gr1+ cells from lungs of aged mice treated with Isotype or anti-IFNAR1 antibody

单核细胞与中性粒细胞是肺部髓系细胞的重要亚群。然而，目前对于衰老如何调控此类肺部细胞，以及I型干扰素(Type I interferon)信号通路的功能尚不清楚。本研究对20~22月龄的老年小鼠开展如下处理：单次腹腔内注射（i.p.）500μg抗IFNAR1抗体（MAR1-5A3克隆株，购自BioXcell公司），或连续注射1周（总计4次，每隔一日注射一次），并于末次注射次日从小鼠肺部分选得到CD45+ CD11b+ Gr1+细胞。随后通过单细胞RNA测序，解析I型干扰素在老年小鼠肺部此类细胞中的作用。本研究采用10x Genomics平台构建单细胞RNA测序（scRNA-seq）文库，以对比经同型对照抗体或抗IFNAR1抗体处理的老年小鼠肺部CD45+ CD11b+ Gr1+细胞之间的基因表达差异。