Dyadic encounter unfamiliar female pairs over 2 days – 16p11.2 Del mouse strain – Del/+-Del/+ - 5171_5164

We evaluated the social interactions and communication between unfamiliar individuals in pairs. For these recordings of social behaviour and ultrasonic communication, we focused on pairs of individuals since we currently cannot identify the emitter of USVs when animals were interacting closely. Therefore, we recorded undisturbed dyadic interactions between two unfamiliar individuals (from two different housing cages) of the same age (14-20 weeks of age) and genotype for 47h (two days and nights). For that purpose, we coupled the Live Mouse Tracker system (plugin 931) with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). LMT and Avisoft systems were synchronised based on the protocol described in (de Chaumont et al., 2021). Each upload includes the sqlite database from LMT (processed, with USV synchronised) and a zip file with all USVs sequences automatically recorded by Avisoft.本研究对成对陌生个体间的二元社交互动与交流行为开展了评估。针对社交行为与超声通讯（ultrasonic vocalizations, USVs）的录制工作，我们仅选择成对个体作为研究对象，原因在于当前无法在动物近距离互动时识别超声发声（USVs）的信号发射者。因此，我们对来自不同饲养笼、年龄一致（14~20周龄）且基因型相同的两只陌生个体进行了为期47小时（含昼夜）的无干扰二元互动录制。为此，我们将活体小鼠追踪系统（Live Mouse Tracker，插件版本931）与1台CM16/CMPA型麦克风（德国格利尼克Avisoft Bioacoustics公司出品）相连，并将该麦克风接入Avisoft Ultrasound Gate 416超声采集设备：采样率300 kHz，采样格式16位；触发参数为基于该通道电平触发，预触发时长1 s，保持时长1 s，有效信号时长＞0.005 s；触发事件判定标准为25~125 kHz频段内能量占比2%且熵值＜50%，该设备同样由德国格利尼克的Avisoft Bioacoustics公司出品。活体小鼠追踪系统（LMT）与Avisoft采集系统依据de Chaumont等人2021年发表的实验方案完成了同步校准。每份上传文件均包含经过处理且完成超声发声（USVs）同步的活体小鼠追踪系统（LMT）SQLite数据库，以及一个包含Avisoft设备自动录制的全部超声发声序列的压缩包。