Supplementary file 5, Significantly differentially expressed genes and transcripts

This study focuses on understanding how plants, specifically Arabidopsis thaliana, respond to two major pollutants, lead (Pb) and vanadium (V) found in oil contaminated land. While the effects of these pollutants on animals are well-studied, their impact on plants is not as clear. To fill this gap, we exposed Arabidopsis seedlings to Pb and V and analyzed the changes in gene expression using advanced sequencing and validated the data using RT PCR technique. The results showed that exposure to Pb and V led to significant changes in gene expression in Arabidopsis seedlings compared to untreated samples. Through Gene Ontology and pathway analysis, we identified several biological processes and pathways associated with the differentially expressed genes. Overall, this investigation sheds light on the molecular mechanisms underlying the toxicity and tolerance of Arabidopsis to Pb and V, providing valuable insights into how plants respond to heavy metal stress.本研究旨在探究植物——尤其是拟南芥（Arabidopsis thaliana）——对石油污染土壤中两种主要污染物铅（Pb）与钒（V）的响应机制。尽管此类污染物对动物的毒性效应已被广泛研究，但其对植物的影响尚未明确。为填补这一研究空白，本研究将拟南芥幼苗暴露于铅与钒环境中，采用高通量测序技术分析其基因表达变化，并通过逆转录PCR（RT PCR）技术对测序数据进行验证。 实验结果表明，与未处理对照组相比，铅与钒暴露会使拟南芥幼苗的基因表达发生显著改变。通过基因本体（Gene Ontology）富集分析与通路富集分析，本研究鉴定出多个与差异表达基因相关的生物学过程及信号通路。综上，本研究阐明了拟南芥应对铅与钒胁迫的毒性耐受分子机制，为解析植物响应重金属胁迫的内在机制提供了宝贵的理论参考。