RNA-Seq analysis miR-455-3p overexpression effect on breast cancer cell line. RNA-Seq analysis miR-455-3p overexpression effect on breast cancer cell line

MiR-455-3p has been reported to suppress the expression of ROCK2, HOXB5, hTERT, and RUNX2, which are involved in multiple biological processes including renal fibrosis, chondrogenic development and differentiation, Alzheimer’s disease, preeclampsia, and tumorigenesis. Here, we showed that miR-455-3p broadly inhibited TGF-beta-induced EMT by repressing the expression of Smad2, ZEB1, and HDAC2 in breast cancer cells, and that miR-455-3p loss-of-function promotes the acquisition of cell invasive properties and the ability to metastasize. These results indicate that miR-455-3p represents an important node that inhibits metastasis of breast cancer. Overall design: MiR-455-3p mimics or controls were transfected into MCF-7 cells (n=3 for each group) for 48 hours. Total RNA was isolated using Trizol reagent (Invitrogen, USA). Agilent 2100 Bioanalyzer (Agilent Technologies, USA) was used to evaluate the RNA quality.

已有研究表明，miR-455-3p（microRNA-455-3p）可抑制ROCK2、HOXB5、hTERT及RUNX2的表达，上述靶基因参与肾纤维化、软骨生成发育与分化、阿尔茨海默病、子痫前期以及肿瘤发生等多种生物学过程。本研究证实，miR-455-3p可通过抑制乳腺癌细胞中Smad2、ZEB1和HDAC2的表达，广泛抑制转化生长因子-β（Transforming Growth Factor-beta, TGF-beta）诱导的上皮间质转化（Epithelial-Mesenchymal Transition, EMT）；且miR-455-3p功能缺失会促进细胞侵袭特性与转移能力的获得。上述结果表明，miR-455-3p是抑制乳腺癌转移的关键调控节点。实验整体设计：将miR-455-3p模拟物（mimics）或对照序列转染至MCF-7细胞（每组设置3个生物学重复），培养48小时后，采用Trizol试剂（Invitrogen，美国）提取总RNA。使用安捷伦2100生物分析仪（Agilent Technologies，美国）评估RNA质量。