June 2003 surface water bacterial abundance at ten Georgia Coastal Ecosystems LTER sampling sites

Surface water samples were collected during low tide surveys near ten Georgia Coastal Ecosystem LTER sampling sites in June, 2003. Unfiltered subsamples were immediately preserved with buffered formalin and refrigerated for transport to the laboratory. The abundance of bacteria in each sample was determined using an epifluorescence microscope by counting DAPI-stained cells retained on a 0.2µm polycarbonate filter. This study was part of the GCE-LTER hydrographic monitoring program, and will be repeated quarterly.

2003年6月，研究人员在退潮采样期间，于佐治亚海岸生态系统长期生态研究（Georgia Coastal Ecosystem LTER）的10个采样点位附近采集了地表水样本。未经过滤的子样本即刻使用缓冲福尔马林进行固定，并冷藏保存以转运至实验室。通过落射荧光显微镜对截留于0.2μm聚碳酸酯滤膜上的DAPI（4',6-二脒基-2-苯基吲哚）染色细胞进行计数，以此测定每份样本中的细菌丰度。本研究为GCE-LTER水文监测计划的组成部分，且将按季度重复实施。