Mechanisms and Fitness Costs of Resistance to Antimicrobial Peptides LL-37, CNY100HL and Wheat Germ Histones. Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

To evolve bacterial resistance to antimicrobial peptides during a simple laboratory experiment. We succeed in isolating antimicrobial peptide (AMP) resistant Salmonella typhimurium LT2 mutants by serially passaging several independent bacterial lineages in progressively increasing concentrations of LL-37, CNY100HL and Wheat Germ Histones. Significant AMP resistance developed in 15/18 independent bacterial lineages. Our data suggests that resistance to antimicrobial peptides can develop rapidly through mechanisms that confer cross-resistance to several AMPs.

本研究旨在通过简易实验室实验诱导细菌演化出对抗菌肽的耐药性。我们通过在逐步升高浓度的LL-37、CNY100HL与小麦胚芽组蛋白（Wheat Germ Histones）中连续传代多个独立细菌谱系，成功分离得到了抗菌肽（antimicrobial peptides，AMP）耐药的鼠伤寒沙门氏菌LT2突变株。18个独立细菌谱系中共有15个演化出了显著的AMP耐药性。本研究数据表明，抗菌肽耐药性可通过赋予对多种抗菌肽交叉耐药性的机制快速产生。