Comparative transcriptome analysis between resistant and susceptible tomato lines uncovers the response mechanism of Cf-16-mediated resistance to Cladosporium fulvum

Leaf mold disease caused by Cladosporium fulvum is a major disease in cultivated tomato plants and affects global tomato production. Some Cf genes, of which Cf-16 is an effective gene for resisting tomato leaf mold, are associated with leaf mold resistance; however, the molecular mechanism is largely unknown. We used comparative transcriptome analysis of C. fulvum-resistant (cv. Ontario7816, including the Cf-16 gene) and C. fulvum-susceptible (cv. Moneymaker) tomato lines to identify differentially expressed genes (DEGs) at 4 and 8 days postinfection with C. fulvum. Our results provide new insights into the resistance response mechanism of Cf genes to C. fulvum, especially the unique characteristics of Cf-16 in response to C. fulvum infection. Overall design: mRNA profiles of C. fulvum-resistant (cv. Ontario7816, including the Cf-16 gene) and C. fulvum-susceptible (cv. Moneymaker) tomato lines were generated by deep sequencing. The sequence reads that passed quality filters were analyzed at the transcript .

由黄枝孢菌（Cladosporium fulvum）引发的番茄叶霉病是栽培番茄的主要病害，对全球番茄产业造成严重冲击。部分Cf基因（Cf genes）与番茄叶霉病抗性相关，其中Cf-16是抗番茄叶霉病的有效基因，但其介导抗性的分子机制仍有待深入解析。本研究对携带Cf-16基因的抗黄枝孢菌番茄品系（品种为Ontario7816）以及感黄枝孢菌番茄品系（品种为Moneymaker）开展比较转录组分析，以鉴定接种黄枝孢菌后第4天和第8天的差异表达基因（differentially expressed genes, DEGs）。本研究结果为解析Cf基因抗黄枝孢菌的抗性响应机制提供了全新视角，尤其阐明了Cf-16在应对黄枝孢菌侵染时的独特分子特征。实验设计概述：通过深度测序获取抗黄枝孢菌番茄品系（品种为Ontario7816，携带Cf-16基因）与感黄枝孢菌番茄品系（品种为Moneymaker）的mRNA表达谱。对通过质量过滤的测序读段进行转录组水平分析。