The collagen, fibrinogen and thrombin biological adhesive is effective in treating experimental liver injuries

ABSTRACT Objective: to evaluate the effectiveness of an collagen-based adhesive associated with fibrinogen and thrombin in experimental liver injuries in rats. Methods: we randomly divided 30 Wistar rats into three groups: A, B and C. All underwent a standard liver traumatic injury. In group A, the lesion was treated with the adhesive; in group B, with conventional, absorbable suture; group C received no treatment. We analyzed the time of hemostasis, mortality, occurrence of adhesions and any histological changes. Results: there was no statistical difference in relation to mortality (p=0.5820). The adhesive treated group showed the lowest hemostasis times (p=0.0573, odds ratio 13.5) and lower incidence of adhesions (p=0.0119). The histological alterations of the Groups A and B were similar, with foreign body granuloma formation separating the adhesive material and the hepatic stroma suture. Conclusion: the collagen adhesive associated with fibrinogen and thrombin was effective in treating experimental hepatic injury, providing a lower incidence of adhesions between the liver and surrounding structures.

摘要 目的：评估联合纤维蛋白原与凝血酶的胶原基粘合剂（collagen-based adhesive）对大鼠实验性肝损伤的治疗效果。方法：将30只Wistar大鼠随机分为A、B、C三组，所有大鼠均接受标准肝创伤造模。其中A组损伤部位采用该粘合剂进行处理，B组采用常规可吸收缝合术处理，C组不予任何干预。本研究对止血时间、死亡率、粘连发生情况及各项组织学变化进行了分析。结果：三组大鼠的死亡率无统计学差异（p=0.5820）。接受粘合剂处理的A组止血时间最短（p=0.0573，优势比为13.5），且粘连发生率更低（p=0.0119）。A组与B组的组织学改变相似，均可见异物肉芽肿形成，将粘合剂材料与肝基质缝合部位分隔开来。结论：联合纤维蛋白原与凝血酶的胶原基粘合剂可有效治疗实验性肝损伤，且能降低肝脏与周围组织间的粘连发生率。