Long-range enhancer-controlled genes are hypersensitive to regulatory factor perturbations Tjalsma et al.

To study the contribution of regulatory factors to long-range enhancer-mediated gene regulation, we created degron cell lines for the factors RAD21, LDB1, and MED23, in human K562 cells. By endogenously tagging these proteins with a degron tag, we were able to quickly deplete them and measure their effect on gene expression using nascent RNA-sequencing. These files contain raw western blot images used to confirm that treatment of these cells with the degron ligand resulted in depletion of the tagged factors.

为探究调控因子在远程增强子介导的基因调控过程中的作用贡献，我们于人类K562细胞中构建了针对RAD21、LDB1及MED23的降解标签（degron）细胞系。通过在这些蛋白的内源基因组位点添加降解标签（degron tag），我们能够快速耗尽靶蛋白，并借助新生RNA测序（nascent RNA-sequencing）技术检测其对基因表达的调控效应。本数据集包含用于验证经降解配体处理细胞后，带有标签的调控因子已被有效耗尽的原始蛋白质免疫印迹（western blot）图像文件。