Analysis of mRNA-miRNA interaction network reveals the role of CAFs-derived exosomes in the immune regulation of oral squamous cell carcinoma [Cal27_mRNA]

To investigate the regulatory effect of exosomes released from CAFs cells on Cal-27 cells after hOMF is activated by PDGF to become CAFs, high-throughput sequencing was performed on co-cultured Cal-27 cells. The RNA-seq data of Cal-27 cells co-cultured with CAFs-Exo or hOMF-Exo were analyzed and compared for gene expression profiling.

为探究经血小板衍生生长因子（platelet-derived growth factor, PDGF）激活并转化为癌相关成纤维细胞（Cancer-associated fibroblasts, CAFs）的人口腔黏膜成纤维细胞（human oral mucosal fibroblasts, hOMF）所分泌的外泌体（exosomes）对Cal-27细胞的调控作用，本研究对共培养的Cal-27细胞进行了高通量测序。随后针对与CAFs来源外泌体（CAFs-Exo）或hOMF来源外泌体（hOMF-Exo）共培养的Cal-27细胞的RNA测序（RNA-seq）数据展开分析，并对比其基因表达谱。