Microarray analysis of embryonic E12.5 mouse gonads from control male, female and Amh; Inhbb dKO males. Microarray analysis of embryonic E12.5 mouse gonads from control male, female and Amh; Inhbb dKO males

Fate determination and maintenance of fetal testes in most mammals occur cell autonomously as a result of the action of key transcription factors in Sertoli cell. In the mouse testis AMH and Activin B are required for the maintenance of Sertoli cell fate. By E12.5, the testis of the dKO male are comparable with the conrol testis as Amh and Activin B are not required for the initial testis differentiation. By E15.5 Sertoli cells at the testis poles in the dKO transdifferentiate into granulosa cells. Overall design: We compared the transcriptional profile of control testis, control ovaries and dKO testis (Amh-/-; Inhbb-/-) testis at E12.5

多数哺乳动物的胎儿睾丸命运决定与维持以细胞自主方式进行，其分子基础为塞尔托利细胞（Sertoli cell）内关键转录因子的调控作用。在小鼠睾丸中，AMH与激活素B（Activin B）是维持塞尔托利细胞命运所必需的因子。在胚胎发育第12.5天（E12.5），双敲除（dKO）雄性小鼠的睾丸与对照睾丸无显著差异，这是因为AMH与激活素B并非睾丸初始分化所必需的调控因子。至胚胎发育第15.5天（E15.5），双敲除小鼠睾丸极性区域的塞尔托利细胞会转分化为颗粒细胞（granulosa cells）。实验设计方案：本研究在胚胎发育第12.5天（E12.5），对对照睾丸、对照卵巢以及双敲除（dKO，即Amh-/-; Inhbb-/-）睾丸的转录组图谱进行了比较分析。