Hyper-repression of HUSH target genes by over-expression of the R252W Charcot-Marie-Tooth disease mutant MORC2

By comparing wild-type SK-N-SH neuroblastoma cells to cells over-expressing either wild-type or R252W mutant MORC2, the goal of the experiment was to assess the effect of MORC2 over-expression on the transcriptome. Overall design: Total RNA-seq of SK-N-SH cells in triplicate, either 1) mock transduced, 2) transduced with lentiviral vector encoding wild-type MORC2 or 3) transduced with lentviral vector encoding R252W MORC2.

本实验通过对比野生型SK-N-SH神经母细胞瘤细胞与分别过表达野生型或R252W突变型MORC2的细胞，旨在评估MORC2过表达对细胞转录组的影响。
总体实验设计：对SK-N-SH细胞进行总RNA测序（Total RNA-seq），每组设置3个生物学重复，共分为三组：1）假转导组；2）转导携带编码野生型MORC2的慢病毒载体（lentiviral vector）组；3）转导携带编码R252W突变型MORC2的慢病毒载体组。