Transcriptome Profiling of CD4+ T cell subpopulations from Aire-Knockout Rats

The dataset consists of microarray gene expression data from four key CD4+
T cell populations—Tconv, Treg, Tfh, and Tfr—isolated from 7-month-old
Aire-knockout rats with high levels of anti-IFNα autoantibodies and
age-matched control rats. The cells were isolated from spleens and
axillary lymph nodes using flow cytometry, with each population being
sorted based on specific markers (CD3+ CD4+ CD25- ICOS- for Tconv, CD25+
ICOS- for Treg, CD25- ICOS+ for Tfh, and CD25+ ICOS+ for Tfr). RNA was
purified from these sorted populations, and transcriptome analysis was
performed using Clariom™ S Pico Assay HT gene expression chips.

本数据集包含4种关键CD4+ T细胞群的微阵列基因表达数据，分别为Tconv、Treg、Tfh及Tfr；这些细胞分离自7月龄的抗IFNα自身抗体水平升高的Aire敲除（Aire-knockout）大鼠，以及同月龄对照大鼠。研究人员通过流式细胞术从脾脏及腋窝淋巴结中分离上述细胞，并基于特定标志物对各细胞群进行分选：Tconv采用CD3+ CD4+ CD25- ICOS-标志物，Treg采用CD25+ ICOS-，Tfh采用CD25- ICOS+，Tfr采用CD25+ ICOS+。从上述分选后的细胞群中纯化RNA，并采用Clariom™ S Pico Assay HT基因表达芯片开展转录组分析。