Identification of WRKY22 targets under submergence in Arabidopsis (mRNA). Arabidopsis thaliana

We analyzed global transcriptional changes in submerged Arabidopsis seedlings comparing control untreated seedlings. The same analysis were also applied on wrky22-ko2 seedling to identify WRKY22 targets under submergence. Overall design: Time course experiments (1, 3, and 6 for Columbia and wrky22-ko2). Tissues from submerged seedlings vs. Tissues from un-submerged seedlings. Biological replicates: 4 replicates for each time point, independently grown, treated, and harvested. One replicate per array. 2 of 4 replicates are dye-swapped.

本研究以未经处理的拟南芥（Arabidopsis）幼苗为对照，分析浸没处理拟南芥幼苗的全转录组全局表达变化。同时针对wrky22-ko2突变体幼苗开展相同分析，以鉴定浸没条件下WRKY22的调控靶标基因。实验设计概述：针对哥伦比亚生态型与wrky22-ko2突变体开展时间进程实验，设置1、3、6三个时间点。实验组为浸没处理幼苗的组织，对照组为未浸没处理幼苗的组织。生物学重复：每个时间点设置4次独立生物学重复，所有材料均独立培养、处理并收获。每个芯片对应一次生物学重复；4次重复中有2次进行了染料互换（dye-swapped）实验。