Expression data of hepatocellular carcinoma and adjacent liver tissues

To identify therapeutic targets with high specificity to HCC, we searched for genes significantly up-regulated in HCC over corresponding non-tumor liver using high-density microarrays Keywords: disease state analysis HCC and surrounding non-tumor liver tissue samples were collected from 10 cases who underwent liver resection for HCC at National Cancer Center Hospital (Tokyo, Japan). One µg of total RNA was converted to end-labeled cRNA using the Whole Transcript Sense Target Labeling kit (Affymetrix, Santa Clara, CA). The fluorescent cRNA probes were hybridized to Human Exon 1.0 ST arrays (Affymetrix), as instructed by the supplier.

为筛选对肝细胞癌（Hepatocellular Carcinoma, HCC）具有高特异性的治疗靶点，本研究利用高密度微阵列技术，筛选在肝细胞癌组织中相较于配对非肿瘤肝组织显著上调的基因。关键词：疾病状态分析。
本研究从日本东京国立癌症中心医院收治的10例行肝细胞癌肝切除术的患者中，采集肝细胞癌组织及其癌旁非肿瘤肝组织样本。
取1μg总RNA，采用全转录义靶标标记试剂盒（Affymetrix，美国加利福尼亚州圣克拉拉市）制备末端标记的互补RNA（cRNA）。
按照试剂盒操作指南，将荧光标记的cRNA探针与人类外显子1.0 ST芯片（Affymetrix）进行杂交。