Figure 6B

A. Schematic representation of the experiment methodology for this figure. B. Supernatants from infected MRC-5 cells expressing either vector or MyD88 at an MOI of 0.05, with TB40/E-mCh, were collected every three days for 15 days and filtered through 0.2 mm disc filter. The filtered supernatants from each day were then added to another monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). Cells were monitored for virus spread by imaging every three days. C. Supernatants from infected MRC-5 cells expressing either vector or MyD88 were collected at 7 dpi and filtered through a 0.2 mm disc filter. They were then either untreated or denatured by boiling at 95° C before storage. The un-boiled and boiled supernatants were added on another monolayer of MRC-5 infected with TB40/E-mCh at MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). The spread of the virus was monitored by imaging every three days. D. A monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 was treated with mock (UT), IFN-β (50ng/ml), or IL1-β (100ng/ml). The spread of the virus was monitored every three days by confocal imaging up to day 15. The spread of infection was quantified as the area of fluorescence using the NIS element software. Results are shown as mean ± SD. * p<0.05.

A. 本图对应实验方法的示意图。
B. 以感染复数（Multiplicity of Infection, MOI）0.05感染TB40/E-mCh，且分别转染空载体或MyD88的MRC-5细胞上清，于15天内每3天收集一次，经0.2 mm圆盘滤器过滤。将每日收集过滤后的上清，每3天添加至另一批以感染复数0.05感染TB40/E-mCh的MRC-5单层细胞中，同时移除孔板内等体积的过滤上清（最终上清与培养基体积比为1:1）。每3天通过成像观测病毒扩散情况。
C. 收集转染空载体或MyD88的TB40/E-mCh感染MRC-5细胞的上清，于感染后第7天（days post infection, dpi）收集并经0.2 mm圆盘滤器过滤。随后将上清分为两组，一组不做处理，另一组于95℃煮沸变性后保存。将未煮沸与煮沸后的上清，每3天添加至另一批以感染复数0.05感染TB40/E-mCh的MRC-5单层细胞中，同时移除孔板内等体积的过滤上清（最终上清与培养基体积比为1:1）。每3天通过成像观测病毒扩散情况。
D. 以感染复数0.05感染TB40/E-mCh的MRC-5单层细胞，分别以空白对照（UT，未处理组）、50ng/ml干扰素β（Interferon-β, IFN-β）或100ng/ml白细胞介素1β（Interleukin-1β, IL-1β）进行处理。每3天通过共聚焦成像观测病毒扩散情况，直至第15天。采用NIS Elements软件通过荧光面积量化病毒感染扩散范围。实验结果以平均值±标准差（Standard Deviation, SD）表示，*标注表示p<0.05。