CIL:46070, Homo sapiens. In Cell Image Library

Plate 26678 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

编号26678的多孔板——表型谱分析（Phenotypic profiling）旨在通过多参数、基于特征的分析手段，对每个样本的细胞表型进行系统性总结，使谱图间的相似性能够反映样本间的相似性。本图像集为评估基于图像的谱分析方法区分小分子化合物效应的能力提供了实验基础。

图像数据集：
本数据集包含经1600种已知生物活性化合物分别处理的U2OS细胞样本，通过六种标记物完成七种细胞器的标记表征，即"细胞绘画"（cell-painting）检测法。

该预实验共包含20块多孔板。每块多孔板设有384个孔位，每个孔位采集9个成像视野，总计69,120个成像视野。每个视野通过5个检测通道（即检测波长）完成成像，每个通道的图像均以独立的灰度图像文件存储，因此本数据集共包含345,600张16位TIFF格式的图像文件。