CP190-HB.2-4 hr OR embryo.Affy 2

modENCODE_submission_5131 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 2-4hr OR; Genotype: wild type; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody CP190-HB (target is CP190); Developmental Stage Embryo 2-4hr OR

modENCODE_submission_5131 本提交内容源自Gary Karpen的modENCODE项目。若需查看modENCODE项目的完整清单，请访问http://www.genome.gov/26524648。
项目目标：我们旨在定位黑腹果蝇（Drosophila melanogaster）全基因组中的125种染色体蛋白。本次研究涉及的蛋白参与DNA复制、基因表达、基因沉默及遗传等基础染色体生物学功能。我们将采用基因组平铺芯片开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。实验初始阶段，我们将使用三种细胞系与两个胚胎阶段的染色质进行蛋白定位分析，后续将把部分蛋白的分析范围拓展至另外四种动物组织/发育阶段。
关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022 与 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：ChIP-chip。
生物来源：菌株：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；发育阶段：Oregon-R品系2-4小时胚胎；基因型：野生型；
生物学重复次数：4次；
实验因素：菌株：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；抗体：CP190-HB（靶标为CP190蛋白）；发育阶段：Oregon-R品系2-4小时胚胎。