Multiomics analyses reveal the mechanical-stress-dependent changes in trabecular meshwork cytoskeletal-extracellular matrix interactions

The purpose of this study was to understand the response of trabecular meshwork under mechanical stretch using a multi-omics approach. Gene expression analysis of trabecular meshwork cells subjected to cyclic mechanical stretch compared to unstretched controls. Primary HTM cell cultures were subjected to 15% stretching at 0.69 Hz frequency for 8 h for mRNA sequencing analysis.本研究旨在通过多组学方法，解析小梁网在机械牵拉作用下的应答反应。本研究对施加周期性机械牵拉的小梁网细胞与未牵拉对照组开展基因表达对比分析。原代人小梁网（HTM）细胞培养物被施以15%拉伸幅度、0.69Hz频率的机械牵拉，持续8小时，以用于mRNA测序分析。