GSE47233: Solexa.SU(HW)-HB.14-16 hr OR embryo

Summary: modENCODE_submission_3958 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 14-16hr OR; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody SU(HW)-HB (target is SU(HW)); Developmental Stage Embryo 14-16hr OR

摘要：modENCODE_submission_3958
本提交源自Gary Karpen主持的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）全基因组范围内的125种染色体蛋白。所研究的蛋白参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。我们将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）技术结合Illumina NGS平台开展实验。实验初期将使用3种细胞系和2个胚胎阶段的染色质进行蛋白定位检测，后续还会将部分蛋白的分析拓展至另外4种动物组织/发育阶段。
关于数据使用的条款与细则，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
整体实验设计：
实验类型：ChIP-seq。
生物样本来源：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；发育阶段：胚胎14-16小时（OR品系）；基因型：野生型。
实验因素：Oregon-R品系（官方命名：Oregon-R-modENCODE，基因型：野生型）；抗体：SU(HW)-HB（靶标为SU(HW)蛋白）；发育阶段：胚胎14-16小时（OR品系）