Loss of tight junction integrity induces loss of epithelial phenotype: case of Claudin11 knock out in testis.. Mus musculus

Epithelium barrier integrity is assumed at least in part by the presence of junctions between cells. Tight-junction formation may be necessary for Sertoli cells to cease dividing and to support germ cell development. Microarray analysis of P20 Cldn11 +/- and Cldn11-/- testes confirmed the impact od Cldn11 deficiency on cell cytoskeleton and junction-related genes. Keywords: transcriptomic analysis Overall design: Two-condition experiment: P20 Cldn11 +/- vs. P20 Cldn11 -/- testes. A pool of RNA from P20 Cldn11 +/- group was used as the reference group. Each P20 Cldn11 -/- was compared to reference group. Three biological replicates were processed in dye swap.

上皮屏障的完整性至少在一定程度上有赖于细胞间连接的存在。紧密连接的形成或许是支持细胞（Sertoli cells）停止增殖并支持生殖细胞发育的必要条件。对出生后第20天（P20）的Cldn11杂合敲除（Cldn11 +/-）与纯合敲除（Cldn11-/-）小鼠睾丸开展的微阵列（Microarray）分析，证实了Cldn11基因缺陷对细胞骨架及连接相关基因的影响。
关键词：转录组分析
实验设计：双条件对照实验，即P20 Cldn11 +/-小鼠睾丸组与P20 Cldn11-/-小鼠睾丸组。将Cldn11 +/-组的RNA混合样本作为参照组，每一份P20 Cldn11-/-小鼠睾丸样本均与该参照组进行比对。实验设置3次生物学重复，并采用荧光染料互换（dye swap）的实验方案。