Differential gene expression in oncostreams and no-oncostreams area in mouse model glioma

Our preliminary data demonstrate that malignant gliomas in both human GBM and mouse models display regular anatomical structures, ‘oncostreams’; we interpret these as a manifestation of GBM self-organization. Oncostreams’ presence correlates with tumor malignancy and parenchymal invasion. Molecular signaling pathways that regulate oncostreams’ organization and function, however, remain unknown. We will test the hypothesis that oncostreams’ present in mouse intracranial glioma model have a characteristic transcriptome that differs from surrounding tumor, and to identify critical genes that regulate oncostreams function. mRNA profiles of laser microdisection samples of oncotreams and no-oncostreams areas.

我们的初步研究数据显示，人类胶质母细胞瘤（Glioblastoma Multiforme, GBM）及小鼠模型中的恶性胶质瘤均呈现规则的解剖结构——“癌流（oncostreams）”；我们将其诠释为胶质母细胞瘤自组织的一种表现形式。癌流的存在与肿瘤恶性程度及脑实质侵袭密切相关。然而，调控癌流结构与功能的分子信号通路仍未明确。本研究将验证下述假说：小鼠颅内胶质瘤模型中的癌流具有区别于周围肿瘤的特征性转录组，并将筛选出调控癌流功能的关键基因。本数据集涵盖癌流区域与非癌流区域的激光显微切割样本的mRNA表达谱。