Eucalyptus xylem diurnal time series

The daily cycle of night and day affects the behaviour and physiology of almost all living things. At the molecular level, many genes show daily changes in expression levels. To determine whether changes in transcript abundance occur in wood forming tissues of Eucalyptus trees we used a cDNA microarray to examine gene expression levels at roughly four hour intervals throughout the day. Experiments were performed using RNA extracted from two biological replicates - GU (Eucalyptus grandis x E. urophylla) and GC (Eucalyptus grandis x camaldulensis) trees. A loop design was used, linking six time points. A dye swap was incorporated to eliminate dye bias.

昼夜交替的日周期节律会影响几乎所有生物的行为与生理机能。在分子层面，诸多基因的表达水平会呈现每日周期性变化。为探究桉树木材形成组织中是否存在转录本丰度的昼夜波动，我们采用cDNA微阵列（cDNA microarray）技术，在全天以约4小时为间隔检测基因表达水平。本实验使用两份生物学重复样本的RNA完成：样本分别取自GU（巨桉×尾叶桉，Eucalyptus grandis x E. urophylla）与GC（巨桉×赤桉，Eucalyptus grandis x camaldulensis）两种桉树植株。实验采用环状设计（loop design）连接六个时间采样点，并引入染料互换（dye swap）步骤以消除染料偏倚。