Genome-wide occupancy of chromatin remodeller SMARCAL1 and BRG1 in human cells

In this study we report genome wide occupancy of SMARCAL1 and BRG1 in control HeLa cells. Our analysis showed binding of these two remodellers on DNA damage response gene, chromatin remodellers, cell cycle and apoptosis regulating genes. Overall design: Chromatin immunoprecipitation of SMARCAL1 and BRG1 was done using polyclonal and monoclonal antibodies respectively and subjected to high-throughput next generation sequencing on illumine platform.

本研究报道了对照组HeLa细胞中SMARCAL1与BRG1的全基因组结合谱。分析结果表明，这两种染色质重塑因子可结合于DNA损伤应答基因、染色质重塑因子编码基因、细胞周期调控基因及凋亡调控基因。
实验设计概述：分别使用多克隆抗体与单克隆抗体对SMARCAL1和BRG1进行染色质免疫共沉淀（Chromatin Immunoprecipitation），随后在Illumina测序平台上开展高通量下一代测序。