five

dBMN_spGAL4_head_posterior: Posterior head confocal imaging of fly driver line dBMN_spGAL4 expressing mCD8::GFP, stained using anti GFP antibodies (green channel) - Autofluorescence from the head cuticle is in the red channel

收藏
DataCite Commons2023-07-28 更新2024-07-13 收录
下载链接:
https://doi.brainimagelibrary.org/doi/10.35077/add-use
下载链接
链接失效反馈
官方服务:
资源简介:
Confocal z-stacks (.tiff stacks) of transgenic driver lines that express in mechanosensory neurons innervating specific bristle populations on the Drosophila melanogaster head. The neurons that are targeted by each driver line were labeled by expressing mCD8::GFP under control of the UAS or LexAop promotors, and then stained using anti-GFP antibodies (green channel). Autofluorescence from the head cuticle is in the red channel. The processed images are published in Figure 3 and Figure 3 – figure supplement 1 of the manuscript “Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila (https://doi.org/10.1101/2023.02.11.528119)”. The confocal stacks used to produce these images are organized based on the driver line used in each figure panel.

本数据集包含黑腹果蝇(Drosophila melanogaster)头部支配特定毛簇群体的机械感觉神经元中表达的转基因驱动品系的共聚焦Z堆叠(.tiff堆叠)图像。每个驱动品系所靶向的神经元通过在UAS或LexAop启动子控制下表达mCD8::GFP进行标记,随后使用抗GFP抗体染色以获取绿色通道信号;果蝇头部表皮的自发荧光对应红色通道信号。经处理的图像已发表于论文《促进黑腹果蝇梳理行为序列的平行感觉通路中的躯体拓扑组织》(https://doi.org/10.1101/2023.02.11.528119)的图3及图3补充材料1。用于生成上述图像的共聚焦Z堆叠图像,已根据每个图面板所使用的驱动品系完成分类整理。
提供机构:
Brain Image Library
创建时间:
2023-07-28
二维码
社区交流群
二维码
科研交流群
商业服务