Gene expression response to glucose/fructose in WT vs. tps1-. Saccharomyces cerevisiae

WT or tps1- yeast grown in galactose were subjected to addition of glucose/fructose; cells were collected for RNA extraction and sequencing to assess the gene expression responses between these treatments Overall design: 2 strains (one wild type and one mutant); 2 treatments per strain; each treatment is an 8-point time-course

将在半乳糖（galactose）中培养的野生型（Wild Type, WT）或tps1基因缺失酵母（tps1- yeast）施加葡萄糖（glucose）与果糖（fructose）处理，随后收集细胞用于RNA提取与测序，以评估不同处理间的基因表达响应差异。实验设计概述：共2株菌株（1株野生型与1株突变株）；每株菌株设置2种处理条件；每种处理条件均包含8个时间点的时间序列实验。