Complete mitogenome and phylogenetic analysis of <i>Brassica oleracea</i> L. var. <i>italica</i> Plenck

In this study, we assembled and annotated the mitochondrial genome (mitogenome) of <i>B. oleracea</i> L. var. <i>italica</i> Plenck. This mitogenome was found to span 219 964 bp, with a GC content of 45.25%. It comprised 61 genes, including 35 protein-coding, 23 transfer RNA, and three ribosomal RNA genes. Among these, only 11 genes contained introns. Codon preference analysis revealed a bias toward codons ending in A/U bases. A phylogenetic analysis demonstrated a close relationship between <i>B. oleracea</i> L. var. <i>italica</i> Plenck, <i>B. oleracea</i> L. botrytis, and <i>B. oleracea</i> var. <i>capitata</i>. This reference mitogenome provides a basis for research on genetic conservation, phylogenetic relationships, and molecular breeding strategies among members of the <i>Brassica</i> genus.本研究组装并注释了西兰花（<i>B. oleracea</i> L. var. <i>italica</i> Plenck）的线粒体基因组（mitogenome）。该线粒体基因组全长219964 bp，GC含量为45.25%。其共包含61个基因，其中包括35个蛋白编码基因、23个转运RNA（transfer RNA）基因以及3个核糖体RNA（ribosomal RNA）基因。在上述基因中，仅11个基因含有内含子。密码子偏好性分析显示，该基因组偏好使用以A/U碱基结尾的密码子。系统发育分析表明，西兰花（<i>B. oleracea</i> L. var. <i>italica</i> Plenck）与花椰菜（<i>B. oleracea</i> L. botrytis）、结球甘蓝（<i>B. oleracea</i> var. <i>capitata</i>）的亲缘关系紧密。本参考线粒体基因组可为芸薹属（<i>Brassica</i>）物种的遗传保护、系统发育关系研究以及分子育种策略研究提供理论基础。