Brazilian ground beef authentication by multiplex polymerase chain reaction

ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.摘要：本研究旨在评估多重聚合酶链式反应（multiplex PCR）在检测通过添加和/或替代水牛肉末方式掺假的市售牛肉末中的效能。本研究制备了三份重复的实验掺假牛肉末样本，并制备了普通牛（Bos taurus）和水牛（Bubalus bubalis）的DNA稀释液，以确定该方法的检测限。同时，从巴西北部不同商铺采集了91份标注为"ground beef"的市售肉类样本，并通过多重聚合酶链式反应进行分析。在17.5%的采集样本中检测到了水牛DNA。研究结果表明，多重聚合酶链式反应是一种高效的检测方法，可在肉样中检出占比10%至100%的水牛肉末掺入物，以及占比0.1%至100%的牛肉掺入物。