Comparison of viromes and microbiomes of 14 pig fecal samples. pig gut microbiotes

The question of the presence of phage encoded antibiotic-resistance genes was revisited here by sampling 14 pig fecal samples in various farms, and performing shotgun, deep sequencing on both total microbiota and highly purified viral fractions. The virome assemblies were sufficiently comprehensive to allow in addition the discovery of two new and prevailing viral taxa.

本研究重新审视了噬菌体编码抗生素抗性基因（phage encoded antibiotic-resistance genes）的存在性问题：我们从多家养殖场采集14份猪粪便样本，并对总微生物群（total microbiota）与高度纯化的病毒组分（highly purified viral fractions）开展鸟枪式深度测序（shotgun deep sequencing）。本次构建的病毒组组装（virome assemblies）覆盖度足够全面，进而额外发现了两个全新且占优势的病毒类群（viral taxa）。