Coupon model for novel rotating-disc rheometry method

The model for the coupons was designed in SolidWorks (Dassault Systèmes); refer to supplementary file 1. Coupons were 3D printed using a Prime 30 PolyJet 3D printer (Objet, Stratasys) using RGD720 photopolymer for the printing material (Stratasys). The coupon was printed at a resolution of 0.0008 inches. The coupon surface was sanded used P300 sandpaper to create a rougher surface for bacteria to attach. Prior to inoculating, coupons were sterilized in 70% ethanol.

该试样（coupon）的模型采用SolidWorks（达索系统，Dassault Systèmes）设计，详见补充文件1。试样采用Prime 30 PolyJet 3D打印机（Objet，Stratasys）进行3D打印，所用打印材料为Stratasys公司生产的RGD720光聚合材料。该试样的打印分辨率为0.0008英寸。为获得更粗糙的表面以利于细菌附着，使用P300砂纸对试样表面进行打磨处理。接种前，需将试样置于70%乙醇中进行灭菌处理。