Expression data from differentiated murine 3T3-L1 pre-adipocytes treated with alpha-linolenic acid (ALA) and delta-6-desaturase (D6D) inhibitor

The goal of this study was to investigate the individual and combined effects of alpha-linolenic acid (the essential omega-3 fatty acid) and an inhibitor of the delta-6 desaturse (SC-26196) during adipocye differentiation. 12 total samples were analyzed. Differential expression analysis was conducted in R with limma, where the following treated samples were compared: control vs ALA, control vs d6d inhibitor, and ALA vs ALA+d6d inhibitor. In each comparison, linear models were fitted with weighted least squares for each transcript cluster. Transcript clusters were ranked through log-odds ratios and un-adjusted p-values with eBayes and topTable with p < 0.01 considered statistically significant.

本研究旨在探究α-亚麻酸（alpha-linolenic acid，即必需omega-3脂肪酸）与δ-6去饱和酶（delta-6 desaturse）抑制剂SC-26196在脂肪细胞分化过程中的单独及联合效应。本研究共分析12个样本。采用R语言结合limma包开展差异表达分析，对比的处理组包括：对照组与ALA组、对照组与δ6d抑制剂组、ALA组与ALA联合δ6d抑制剂组。在每一组对比中，均针对每个转录本簇（transcript cluster）拟合加权最小二乘线性模型。借助eBayes与topTable，通过对数优势比与未校正p值对转录本簇进行排序，以p<0.01作为统计学显著性判定阈值。