Investigation of ripk3-dependent IAV-induced genes

Ripk3-deficient fibroblasts and lung epithelial cells are resistant to influenza-induced cell deaths. However, Ripk3 is required for protection against influenza infection in vivo. Here, we examine the influenza-regulated gene expressions between wild type and knock out MEFs as well as an involvement of kinase activity if any. Ripk3-/- or Ripk3+/+ MEFs treated with inhibitor of RIPK3 infected with PR8-ΔNS1 virusRipk3缺陷的成纤维细胞与肺上皮细胞可抵抗流感诱导的细胞死亡。然而，在体内对抗流感感染的免疫保护过程中，Ripk3是不可或缺的。本研究检测了野生型与Ripk3敲除小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts, MEFs）中流感调控的基因表达，并探究了激酶活性是否参与其中。实验中，我们使用RIPK3抑制剂处理Ripk3-/-或Ripk3+/+ MEFs，随后以PR8-ΔNS1病毒感染上述细胞。