Physical characteristics of DIA1 proteins and similarity to orthologues from key species.

aDIA1 homologues were restricted to the Metazoa, but were not found in the phylum Porifera or Nematoda. Partial homologues, including those found in the phylum Mollusca and Annelida (Table S2), are not shown. See Table S1 for accession numbers of full length DIA1 proteins. Only a single Drosophila species DIA1 is included here. Table S3 is an expanded version of this Table.
bIsoelectric point calculated using the assumption that all residues have pKa values equivalent to that of isolated residues, and so may not accurately represent the value for the folded protein.
cIsotopically-averaged molecular weight prediction in kiloDaltons.
dThe BLASTP E-value measures the statistical significance threshold for protein sequence matches. The smaller the number, the better the match. Computer shorthand nomenclature is used to present E-values when values are small. For example, 5e-01 = 0.5 and 5e-04 = 0.0005. Values below 1e-250 are indicated as zero, and details of those greater than 10 are not provided. A dash is used when protein alignments have 100% identity.
eProteins were compared to DIA1from H. sapiens, C. intestinalis, S. purpuratus, or D. melanogaster, or N. vectensis by protein BLAST. The DIA1 proteins used for comparison were chosen as representives from Class Mammalia, Class Aves, and Class Neopterygii within the subphylum Vertebrata, subphylum Urochordata, phylum Echinodermata, phylum Arthropoda, and phylum Cnidaria, respectively.

a DIA1同源物仅分布于后生动物界（Metazoa），但未在多孔动物门（Porifera）与线虫动物门（Nematoda）中检出。部分同源物（如软体动物门、环节动物门的同源物，详见补充表S2）未在本表格中展示。全长DIA1蛋白的登录号信息请参见补充表S1。本文仅纳入了1种果蝇属物种的DIA1蛋白。本表格的扩展版本详见补充表S3。
b 等电点（isoelectric point）的计算基于所有氨基酸残基的pKa值与游离残基一致的假设，因此该计算结果可能无法准确反映折叠后蛋白质的实际等电点数值。
c 同位素平均分子量预测值，单位为千道尔顿（kiloDaltons）。
d BLASTP E值（BLASTP E-value）用于衡量蛋白质序列比对匹配的统计学显著性阈值：数值越小，匹配度越高。当E值极小时，采用计算机科学常用的简写格式表示，例如5e-01代表0.5，5e-04代表0.0005。E值低于1e-250时记为0，E值大于10的结果未在此处提供详细信息。若蛋白质序列比对的序列同一性为100%，则以短横线（-）表示。
e 本研究通过蛋白质BLAST（protein BLAST）将待测蛋白与以下物种的DIA1蛋白进行比对：智人（H. sapiens）、玻璃海鞘（C. intestinalis）、紫球海胆（S. purpuratus）、黑腹果蝇（D. melanogaster）以及星状海葵（N. vectensis）。本次比对选用的DIA1蛋白分别代表脊椎动物亚门（Vertebrata）中的哺乳纲（Mammalia）、鸟纲（Aves）和新鳍鱼纲（Neopterygii），以及尾索动物亚门（Urochordata）、棘皮动物门（Echinodermata）、节肢动物门（Arthropoda）与刺胞动物门（Cnidaria）的代表类群。