Data Sheet 1_Concurrent training and intermittent fasting reduce transverse cross-sectional area of cardiomyocytes and body mass in Wistar rats.pdf

MethodsThis study identified the alterations caused by concurrent training and intermittent fasting on the myocardium of rats. In total, 39 adult male Wistar rats were used, divided into four groups: control [C (n = 12)], fasting control [FC (n = 11)], training [T (n = 8)], and fasting training [FT (n = 8)]. The critical load test was performed to evaluate and determine the intensity of effort in aerobic training (AT) (swimming). The resistance training protocol (RT) (anaerobic) consisted of four series of 10 jumps, with overload corresponding to 50% of the body mass of each animal, and 1 min of rest between each series. The concurrent training (CT) was composed of a protocol of AT and RT in the same session. The intermittent fasting period was 12/12 h. At the end of the experiment, the animals were weighed to obtain the Lee index; heart weight was verified, and tissue samples were collected for further histological analysis. After obtaining the data, the Shapiro-Wilk test was performed, followed by a two-way analysis of variance (ANOVA) with Tukey’s post-test for the variables measured at the end of the experiment.
ResultsFor the variables body mass, feed consumption, and the anaerobic threshold, the FC group showed a greater decrease in the Lee index [(0.28 ± 0.00) p < 0.05)] in relation to the other groups. There were no significant alterations (p > 0.05) in relation to heart weight, fractal dimension, and anaerobic threshold (AnT); finally, both the T and the FT groups presented a significant decrease ([T: (277.3 ± 119.3/FT: 310.5 ± 148.8] p < 0.05) in the transverse cross-sectional area of the cardiomyocytes, when compared to the control groups.
ConclusionThe practice of concurrent training for a period of 4 weeks precipitated a decrease in the transverse cross-sectional area of the cardiomyocytes of Wistar rats. Furthermore, when combined with intermittent fasting, concurrent training not only led to a reduction in the transverse cross-sectional area of the cardiomyocytes but also resulted in a decrease in body mass compared to the isolated concurrent training model.

方法 本研究明确了复合训练（concurrent training, CT）与间歇性禁食对大鼠心肌造成的改变。本次实验共使用39只成年雄性Wistar大鼠，分为四组：对照组[C (n=12)]、禁食对照组[FC (n=11)]、训练组[T (n=8)]以及禁食训练组[FT (n=8)]。通过临界负荷测试评估并确定有氧训练（aerobic training, AT，游泳）的运动强度。抗阻训练方案（resistance training, RT，无氧训练）包含4组10次跳跃，负重为每只动物体质量的50%，每组间休息1分钟。复合训练（concurrent training, CT）方案为单次训练中结合有氧训练与抗阻训练。间歇性禁食周期为12/12小时。实验结束后，称量大鼠体质量以计算Lee指数；测定心脏重量，并采集组织样本用于后续组织学分析。获取实验数据后，首先进行Shapiro-Wilk检验，随后针对实验结束时测得的各项变量采用双因素方差分析（ANOVA）结合Tukey事后检验。结果 针对体质量、摄食量与无氧阈值（anaerobic threshold, AnT）变量，禁食对照组（FC）的Lee指数降幅显著高于其余各组[(0.28±0.00)，p<0.05]。心脏重量、分形维度与无氧阈值未出现显著改变（p>0.05）；最终，与对照组相比，训练组（T）与禁食训练组（FT）的心肌细胞横截面积均出现显著降低[T: (277.3±119.3)/FT: 310.5±148.8]，p<0.05。结论 为期4周的复合训练可导致Wistar大鼠心肌细胞横截面积减小。此外，复合训练与间歇性禁食联合应用时，不仅可使心肌细胞横截面积减小，相较于单纯复合训练模型，还可进一步降低大鼠体质量。