Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c39_h1930004_CaB. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - m3c39_h1930004_CaB

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snm3C-seq to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞构筑，对于理解其多样功能与复杂的动物行为至关重要。我们对人类大脑的多个脑区开展了单细胞核甲基化测序（single nucleus methylation sequencing），鉴定得到多个不同的细胞簇，并构建了其层级分类系统。这些细胞簇涵盖了已被确认的主要脑细胞类型以及潜在的细胞亚型。我们利用这些数据解析了各细胞簇的表观基因组特征，并明确了每一类细胞簇的特异性调控元件。整体实验设计：对脑区开展snm3C-seq测序，以解析成年人类大脑的表观基因组图谱（epigenomic landscape）。