Transcriptome profiles of differentiated mouse embryonic stem cells cultured in normal condition and static magnetic field condition by RNA-seq analysisanalysis. Transcriptome profiles of differentiated mouse embryonic stem cells cultured in normal condition and static magnetic field condition by RNA-seq analysisanalysis

Embryonic stem cells (ESCs) have the ability to differentiate into cells of the three germ layers, and leukemia inhibitory factor (LIF) maintains the pluripotency and promotes the proliferation of ESCs. In the absence of LIF, ESCs spontaneously differentiate and form three-dimensional aggregates known as embryoid bodies (EBs). The differentiation of EBs mimics the process of embryonic development, that is, the differentiation of cells into the three embryonic germ layers (endoderm, mesoderm, and ectoderm), some of which differentiate into beating cardiomyocytes. Static magnetic fields have diverse effects on organisms, studies on the regulation of the differentiation of ESCs to cardiomyocytes by static magnetic fields are not sufficient. To better understand transcriptional landscape and signal transductions, we performed RNA-seq analysis of EBs cultured in two different conditions: conventional incubator, static magnetic field incubator. Overall design: We sought to investigate the differentiated expressed genes of J1 ESCs-derived differentiated cells cultured in two conditions: conventional incubator (short as NC), static magnetic field incubator (short as MF). EBs with biological triplicates were collected for RNA extraction and RNA-seq analysis simultaneously.

胚胎干细胞（Embryonic Stem Cells, ESCs）具备分化为三胚层细胞的潜能，白血病抑制因子（Leukemia Inhibitory Factor, LIF）可维持其多能性并促进增殖。在缺乏LIF时，胚胎干细胞会自发分化形成三维聚集体，即拟胚体（embryoid bodies, EBs）。拟胚体的分化过程模拟胚胎发育进程：细胞可分化为内胚层、中胚层与外胚层三个胚胎胚层，其中部分细胞会进一步分化为搏动型心肌细胞。静磁场对生物体具有多样的生物学效应，但目前关于静磁场调控胚胎干细胞向心肌细胞分化的研究仍较为不足。为更好地解析转录组景观与信号转导通路，我们对两种培养条件下的拟胚体开展了RNA测序（RNA-seq）分析：常规培养箱与静磁场培养箱。实验整体设计：本研究旨在探究两种培养条件下J1胚胎干细胞来源的分化细胞的差异表达基因：常规培养组（简记为NC）、静磁场培养组（简记为MF）。我们同时收集了具有生物学重复的拟胚体样本，用于RNA提取及RNA-seq分析。