Single-nuclei RNA sequencing analysis from a WT mouse brain cortex after Intrathecal(IT) injection of AAV9/JeT-hSLC6A1 vector

Single-nuclei RNA sequencing analysis conducted at 6 months old from a WT mouse brain cortex, following Intrathecal(IT) injection of AAV9/JeT-hSLC6A1 vector at PND10. Overall design: WT C57BL/6J mouse was IT injected with 5 µL AAV9/ JeT-SLC6A1 vector at postnatal 10 days old. At 6 months post-injection, treated mouse was perfused, the brain cortex tissue was collected and processed for Single-nuclei RNA sequencing analysis (10x Genomics)

本数据集包含针对出生后第10天（Postnatal Day 10, PND10）经鞘内（Intrathecal, IT）注射AAV9/JeT-hSLC6A1载体的野生型（Wild Type, WT）小鼠大脑皮层，于感染后6月龄时开展的单细胞核RNA测序（Single-nuclei RNA sequencing）分析。实验设计：于出生后第10天，向野生型C57BL/6J小鼠鞘内注射5 μL AAV9/JeT-SLC6A1载体；感染后6月龄时，对该处理小鼠进行灌流取材，收集大脑皮层组织并通过10x Genomics平台完成单细胞核RNA测序分析。