Expression data of Arabidopsis pollen tube of an AGP6 AGP11 double null mutant. Arabidopsis thaliana

Arabinogalactan proteins are proteoglycans known to have important roles during cell growth and development, namelly during pollen tube growth. A microarray experiment was performed on agp6 agp11 pollen tubes to search for genetic interactions in the context of pollen tube growth. Overall design: RNA from wt and mutant pollen tubes was extracted after 8h of in vitro germination and hybridized on Affimetrix microarrays.

阿拉伯半乳聚糖蛋白（Arabinogalactan proteins, AGPs）是一类蛋白聚糖，已知其在细胞生长与发育过程中发挥关键作用，尤其参与花粉管生长过程。为探究花粉管生长背景下的遗传互作机制，本研究针对agp6与agp11突变体的花粉管开展了微阵列（microarray）实验。实验设计概述：体外萌发8小时后，分别提取野生型（wild type, WT）与突变体花粉管的总RNA，将其与Affymetrix微阵列芯片进行杂交。