SM001: SARS CoV MA15 infection of C57Bl/6 mouse model – Data from 4 viral doses at 1, 2, 4 and 7 days post infection.

Purpose of experiment was to perform transcriptomic analysis on C57Bl/6 mice infected with different doses of SARS CoV MA15 at 4 different days post infection. Twenty-week-old C57BL/6 mice were infected by intranasal instillation of 10^2, 10^3, 10^4 or 10^5 PFU of SARS CoV MA15 in 50 µl of PBS or mock-infected with PBS alone. At days 1, 2, 4 and 7 days post-infection, lungs were harvested and total RNA was isolated and subjected to microarray analysis. The experimental design was for 4- 5 mice/time point/dose and 3 time-matched mocks. The NIAID Systems Virology Center

本实验旨在对感染不同剂量严重急性呼吸综合征冠状病毒MA15（SARS CoV MA15）的C57BL/6小鼠，在感染后4个时间点开展转录组学分析。20周龄的C57BL/6小鼠通过鼻内滴注50 μL磷酸盐缓冲液（PBS）中含10²、10³、10⁴或10⁵噬斑形成单位（PFU）的SARS CoV MA15进行感染，或以单纯PBS进行假感染。分别于感染后第1、2、4、7天采集肺部组织，提取总RNA并进行微阵列（microarray）分析。本实验设计为每个剂量/时间点设置4~5只小鼠，同时设置3只时间匹配的假感染对照组。美国国家过敏与传染病研究所（NIAID）系统病毒学中心