Expression analysis of tomato S. pimpinellifolium accession LA1589 wild type and the Nearly Isogenic Lines lc, fas and lc/fas NILs during floral development.

The goal of this study is to decipher the effect of the tomato lc and fas mutation on gene expression during floral development in tomato. The 3'Tag RNA libraries were sequenced on a Illumina HiSeq2500 at Genomic Resources Core Facility at Weill Cornell Medical College for 100 bp single–end sequencing. Total raw reads for each sample ranged between 5 to 10 million per sample. Overall design: For each genotype, samples from 5-7 week old seedlings were collected in triplicate in the greenhouse and immediately stored in RNAlater. Using the dissecting microscope, sympodial shoot apical meristem (SYM), the combined floral and inflorescence meristem (F&IM), and floral buds at 2, 4, and 6 days post floral initiation (dpi) were collected and frozen in the -80 C.

本研究旨在阐明番茄lc与fas突变对番茄花发育过程中基因表达的影响。3'标签RNA文库（3'Tag RNA libraries）于威尔·康奈尔医学院基因组资源核心设施（Genomic Resources Core Facility），采用Illumina HiSeq2500测序平台完成100 bp单端测序。每个样本的原始测序读段总量介于500万至1000万之间。
实验整体设计如下：针对每个基因型，于温室中采集5至7周龄幼苗样本，设置三次生物学重复，并立即置于RNAlater中保存。借助解剖显微镜，分别采集合轴茎尖分生组织（SYM）、花与花序分生组织联合体（F&IM），以及花起始后2、4、6天的花芽（dpi）样本，随后置于-80℃冰箱中速冻保存。